微信公众号

官网二维码
中国癌症防治杂志

中国癌症防治杂志 ›› 2014, Vol. 6 ›› Issue (2): 109-114.doi: 10.3969/j.issn.1674-5671.2014.02.03

• 基础研究 • 上一篇    下一篇

PRX2和AKR1B10在二阶段化学诱发小鼠肝癌形成过程中的表达及意义

  

  1.  广西肿瘤防治研究所实验研究部；广西医科大学研究生学院
  • 出版日期:2014-06-25 发布日期:2014-07-08
  • 通讯作者: 谢裕安 E-mail:gxwskj@163.com
  • 基金资助:

    国家自然科学基金资助项目（81060203）

Expression and significance of PRX2 and AKR1B10 during chemically induced hepatocarcinogenesis in mice

  • Online:2014-06-25 Published:2014-07-08

PDF (PC)

600

赞

摘要： 目的 探讨PRX2和AKR1B10在二阶段化学诱发小鼠肝癌形成过程中的表达及意义。方法 90只C57BL/6J雄性小鼠随机分为实验组（60只）和正常对照组（30只），用DEN/CCl4 /乙醇诱发小鼠肝癌。于第4周、第8周、第12周、第16周和第20周5个时点处死小鼠，观察其肝脏病理组织的改变，应用实时荧光定量PCR法、免疫组化SP法分别检测小鼠肝组织PRX2 mRNA和AKR1B10 mRNA及蛋白质的表达水平。结果 ①实验诱发小鼠肝癌过程中的第4周见肝细胞肿胀，炎细胞浸润，肝脏呈炎症病变；第8周见肝组织炎症病变加重，出现纤维组织增生及肝细胞再生；第12周见肝组织呈不典型增生；第16周有典型假小叶形成；第20周可见典型的小鼠肝癌病理改变。②化学诱癌的第4周至第20周实验组小鼠肝组织PRX2 mRNA和AKR1B10 mRNA和蛋白质的表达均高于正常对照组（P<0.05），且两者的表达水平随着时间的延长，均呈逐步升高的趋势；至第20周肝癌形成时，实验组两者的表达均显著高于癌前各时间点（P<0.05）。③在二阶段化学诱发小鼠肝癌形成过程中，PRX2 mRNA和AKR1B10 mRNA的表达水平呈正相关（r=0.674，P=0.05）。结论 在二阶段化学诱发小鼠肝癌形成过程中PRX2和AKR1B10的高表达是化学诱发小鼠肝癌形成的早期事件，PRX2和AKR1B10可能在化学诱发小鼠肝癌的发生、发展和促进过程中起重要作用。

关键词: 肝肿瘤, 小鼠肝癌, C57BL/6J小鼠, PRX2, AKR1B10

Abstract: Objective To explore the expression and significance of PRX2 and AKR1B10 during chemically induced hepatocar-cinogenesis in mice. Methods Primary hepatocellular carcinoma（HCC）was induced in 60 C57BL/6J mice by exposing them to a combination of diethylnitrosamines（DEN），carbon tetrachloride（CCl4）and ethanol. Another group of 30 mice received no drugs and served as a negative control.Mice were killed on weeks 4，8，12，16 and 20 and tissue samples were harvested for staining with hematoxylin and eosin.Samples were also analyzed by QPT-PCR and immunohistochemistry to detect changes in PRX2 and AKR1B10 mRNA and protein. Results In mice with chemically induced HCC，toxic hepatitis and acute hepatocellular necrosis were observed by week 4.By week 8，hepatocellular necrosis had gotten worse and liver fibrosis was detectable.Atypical hyperplasia was observable by week 12，pseudolobule by week 16 and hepatocellular carcinoma by week 20.Expression of PRX2 and AKR1B10 was significantly higher in the HCC animals than in control animals from week 4 to 20（P<0.05），and levels continued to increase with time.Levels were significantly higher in the HCC animals by week 20 than before chemical induction of HCC（P<0.05）.Expres-sion of PRX2 mRNA positively correlated with that of AKR1B10 mRNA（r=0.674，P=0.05）. Conclusion Overexpression of PRX2 and AKR1B10 may be early events during chemically induced hepatocarcinogenesis in mice and may promote hepatocarcinogenesis.

Key words: Liver neoplasm, Hepatocarcinogenesis in mice, C57BL/6J mice, PRX2, AKR1B10